human leptin duoset elisa  (Bio-Techne corporation)

Journal: iScience

Article Title: Development of an adipose-tropic AAV capsid ablating liver tropism

doi: 10.1016/j.isci.2024.110930

Figure Lengend Snippet: Intraperitoneal administration of V7-LEP/ADIPOQ vector rescues the excessive weight gain and impaired glycemic control in ob/ob mice (A) Schematic of the AAV vector containing human leptin and human adiponectin linking by 2A sequence. (B) Body weight. (C) Area under the curve of body weight. (D) Weight gain. (E) Area under the curve of weight gain. (F) Glucose tolerance test at 8-week post AAV injection. (G) Area under the curve of glucose tolerance test. Data are means ± SEM. Sample size (biological replicates): ob /+ buffer n = 5, ob/ob GFP 8E10 n = 5, ob/ob LEP/ADIPOQ 4E10 n = 4, ob/ob LEP/ADIPOQ 8E10 n = 4. ∗ p < 0.05, ∗∗ p < 0.01 from one-way ANOVA followed by Tukey’s multiple comparison test.

Article Snippet: Human Leptin ELISA , R&D System , Cat#DY398.

Techniques: Plasmid Preparation, Control, Sequencing, Injection, Comparison

Journal: iScience

Article Title: Development of an adipose-tropic AAV capsid ablating liver tropism

doi: 10.1016/j.isci.2024.110930

Figure Lengend Snippet: Intraperitoneal administration of V7-LEP/ADIPOQ reverses hyperinsulinemia, hyperglycemia, and hepatic steatosis in ob/ob mice (A) Serum levels of human leptin and human adiponectin. No significant difference between the low dose and high dose. (B) Serum level of insulin. (C) Serum level of glucose. (D) Liver triglyceride content. Data are means ± SEM. Sample size (biological replicates): ob /+ buffer n = 5, ob/ob GFP 8E10 n = 5, ob/ob LEP/ADIPOQ 4E10 n = 4, ob/ob LEP/ADIPOQ 8E10 n = 4. ∗ p < 0.05, ∗∗ p < 0.01 from One-way ANOVA followed by Tukey’s multiple comparison test.

Article Snippet: Human Leptin ELISA , R&D System , Cat#DY398.

Techniques: Comparison

Journal: iScience

Article Title: Development of an adipose-tropic AAV capsid ablating liver tropism

doi: 10.1016/j.isci.2024.110930

Figure Lengend Snippet:

Article Snippet: Human Leptin ELISA , R&D System , Cat#DY398.

Techniques: Recombinant, SYBR Green Assay, Protease Inhibitor, Enzyme-linked Immunosorbent Assay, Colorimetric Assay, Mutagenesis, Reverse Transcription, Software

Journal: iScience

Article Title: Development of an adipose-tropic AAV capsid ablating liver tropism

doi: 10.1016/j.isci.2024.110930

Figure Lengend Snippet: Intraperitoneal administration of V7-LEP/ADIPOQ vector rescues the excessive weight gain and impaired glycemic control in ob/ob mice (A) Schematic of the AAV vector containing human leptin and human adiponectin linking by 2A sequence. (B) Body weight. (C) Area under the curve of body weight. (D) Weight gain. (E) Area under the curve of weight gain. (F) Glucose tolerance test at 8-week post AAV injection. (G) Area under the curve of glucose tolerance test. Data are means ± SEM. Sample size (biological replicates): ob /+ buffer n = 5, ob/ob GFP 8E10 n = 5, ob/ob LEP/ADIPOQ 4E10 n = 4, ob/ob LEP/ADIPOQ 8E10 n = 4. ∗ p < 0.05, ∗∗ p < 0.01 from one-way ANOVA followed by Tukey’s multiple comparison test.

Article Snippet: R&D Systems DuoSet ELISA kits were used to assay serum human leptin (R&D Systems, #DY398) and human adiponectin/Acrp30 (R&D Systems, # DY1065).

Techniques: Plasmid Preparation, Control, Sequencing, Injection, Comparison

Journal: iScience

Article Title: Development of an adipose-tropic AAV capsid ablating liver tropism

doi: 10.1016/j.isci.2024.110930

Figure Lengend Snippet: Intraperitoneal administration of V7-LEP/ADIPOQ reverses hyperinsulinemia, hyperglycemia, and hepatic steatosis in ob/ob mice (A) Serum levels of human leptin and human adiponectin. No significant difference between the low dose and high dose. (B) Serum level of insulin. (C) Serum level of glucose. (D) Liver triglyceride content. Data are means ± SEM. Sample size (biological replicates): ob /+ buffer n = 5, ob/ob GFP 8E10 n = 5, ob/ob LEP/ADIPOQ 4E10 n = 4, ob/ob LEP/ADIPOQ 8E10 n = 4. ∗ p < 0.05, ∗∗ p < 0.01 from One-way ANOVA followed by Tukey’s multiple comparison test.

Article Snippet: R&D Systems DuoSet ELISA kits were used to assay serum human leptin (R&D Systems, #DY398) and human adiponectin/Acrp30 (R&D Systems, # DY1065).

Techniques: Comparison

Journal: iScience

Article Title: Development of an adipose-tropic AAV capsid ablating liver tropism

doi: 10.1016/j.isci.2024.110930

Figure Lengend Snippet:

Article Snippet: R&D Systems DuoSet ELISA kits were used to assay serum human leptin (R&D Systems, #DY398) and human adiponectin/Acrp30 (R&D Systems, # DY1065).

Techniques: Recombinant, SYBR Green Assay, Protease Inhibitor, Enzyme-linked Immunosorbent Assay, Colorimetric Assay, Mutagenesis, Reverse Transcription, Software

Journal: Heliyon

Article Title: Obesity alters adipose tissue response to fasting and refeeding in women: A study on lipolytic and endocrine dynamics and acute insulin resistance

doi: 10.1016/j.heliyon.2024.e37875

Figure Lengend Snippet: Key resources table

Article Snippet: Human leptin ELISA DuoSet Kit , R&D systems Biotechne , Cat# DY398.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Software

Journal: Heliyon

Article Title: Obesity alters adipose tissue response to fasting and refeeding in women: A study on lipolytic and endocrine dynamics and acute insulin resistance

doi: 10.1016/j.heliyon.2024.e37875

Figure Lengend Snippet: Key resources table

Article Snippet: Human leptin ELISA DuoSet Kit , R&D systems Biotechne , Cat# DY398.

Techniques: Clinical Proteomics, Recombinant, Enzyme-linked Immunosorbent Assay, Software